Molecular Docking and In-Silico Analysis of Natural Biomolecules against Dengue, Ebola, Zika, SARS-CoV-2 Variants of Concern and Monkeypox VirusArticle Published on 2022-09-222022-11-15 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, 진단, 치료제, [키워드] Amino acid AMP AMPs antimicrobial peptide Antiviral antiviral drug Antiviral treatment AutoDock vina B.1.617.2 Bacterial bacterial AMPs binding energy Biomolecule biomolecules Cell cell receptor cell surface receptors concern Control Dengue dengue virus determine develop docking docking score Ebola Ebola virus Effective vaccines envelope protein Evolution in silico increase in indicated Modification molecular molecular docking Monkeypox monkeypox. mutant natural Occurrence P.1 pathogenic viruses positive proline Protein receptors SARS-CoV-2 Secondary Metabolite specific neutralizing antibody tested the SARS-CoV-2 the spike protein therapeutic agents variant variants of SARS-CoV-2 viral cell viral surface virus virus was performed Zika Zika virus [DOI] 10.3390/ijms231911131 PMC 바로가기
Spike Protein Cleavage-Activation in the Context of the SARS-CoV-2 P681R Mutation: an Analysis from Its First Appearance in Lineage A.23.1 Identified in UgandaArticle Published on 2022-08-312022-11-16 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, [키워드] A.23.1 activated Activation Affect Alpha variant analyzed appearance arginine B.1.1.7 B.1.617 B.1.617.2 cell-cell fusion change changes changes in cleavability cleavage Context Course delta variant functional functional consequences furin furin cleavage site host cell Identified increase in infectivity assay less Lineage mimicking Mutation natural selection notable occur occurred P681R P681R mutation pandemic Particle Pathogenesis peptide performed position 681 predicted proline protease pseudotyped S1/S2 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike significantly increased spike spike gene Spike protein Spike. Spread Surveillance the SARS-CoV-2 Transmissibility transmitted Uganda variant viral variant virus virus [DOI] 10.1128/spectrum.01514-22 PMC 바로가기
A highly efficacious live attenuated mumps virus-based SARS-CoV-2 vaccine candidate expressing a six-proline stabilized prefusion spike6-프롤린 안정화된 융합전 스파이크를 발현하는 매우 효과적인 약독화성 볼거리 바이러스 기반 SARS-CoV-2 백신 후보Article Published on 2022-08-162022-09-11 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] antibody backbone candidate children COVID-19 vaccine Delta delta variant Delta variants effective expressed expressing expression Genome hamster human vaccine IFNAR1 immune responses Immunity immunization immunogenic Immunoglobulin immunoglobulin A include Infant Infants intranasal junction mice MMR MMR vaccine mucosal mump mumps virus neutralized Neutralizing antibodies neutralizing antibody prefusion proline prolines raised rapid increase Safe SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 USA-WA1/2020 SARS-CoV-2 vaccine SARS-CoV-2 variant SARS-CoV-2 variants sera Spike protein T cell tetravalent Vaccine vaccine strain vaccine. variants virus [DOI] 10.1073/pnas.2201616119 PMC 바로가기 [Article Type] Article
A circular mRNA vaccine prototype producing VFLIP-X spike confers a broad neutralization of SARS-CoV-2 variants by mouse seraVFLIP-X 스파이크를 생성하는 원형 mRNA 백신 프로토타입은 마우스 혈청에 의한 SARS-CoV-2 변이체의 광범위한 중화를 제공합니다Article Published on 2022-08-012022-09-11 Journal: Antiviral Research [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, [키워드] addition Amino acid amino acid sequence amino acids Antibody titers B.1.1.529 B.1.617.2 balance BNT162b2 boost cellular immune responses cleavage site COVID-19 vaccine Critical D614G detectable Disulfide disulfide bond E484K Evolution of SARS-CoV-2 FIVE flexible glycosylation greater humoral Humoral and cellular immune responses immune responses immunization immunized induce K417N L452R linear linker mice mRNA mRNA vaccine mRNA vaccines mRNA-1273 N501Y neutralization Neutralizing activity Neutralizing antibody response Neutralizing antibody titer neutralizing antibody titers offer pre-fusion producing proline proline substitutions prolines reduced response S-2P SARS-CoV-2 SARS-CoV-2 vaccine SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV-2 virus sequence sera shown spike Spike protein spike trimer stability suggested T478K the spike protein transcript trimer trimeric Vaccine vaccine. Vaccines variant variants variants of concern variants of interest VFLIP virus VOCs VOIs wildtype [DOI] 10.1016/j.antiviral.2022.105370 PMC 바로가기 [Article Type] Article
Heterologous saRNA Prime, DNA Dual-Antigen Boost SARS-CoV-2 Vaccination Elicits Robust Cellular Immunogenicity and Cross-Variant Neutralizing Antibodies이종 saRNA 프라임, DNA 이중 항원 부스트 SARS-CoV-2 백신 접종으로 강력한 세포 면역원성과 교차 변형 중화 항체 유도Article Published on 2022-07-152022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료법, [키워드] Adenovirus animal model animal models B.1.617.2 Beta boost CD4+ CD8+ T-cell combinations COVID-19 D614G mutation Delta distribution DNA dual dual antigen ENCODE enhanced expression followed by furin furin cleavage region Glutamine Heterologous highest homologous homologous or heterologous Humoral response humoral responses IgG immune response immune responses immunogenicity lipid carrier MHC class I MHC class I and II mice motif N antigen Neutralizing nucleic acid nucleocapsid omicron peptide peptides prefusion conformation prime proline prolines protease pseudovirus receiving regimen regimens Region response RNA vaccine SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant self-amplifying RNA sequence sera serotype spike Stimulation strain Strains Support T-cell T-cell responses vaccination Vaccine vaccine. Vaccines variant wild type Wuhan [DOI] 10.3389/fimmu.2022.910136 PMC 바로가기 [Article Type] Article
Urine metabolomics links dysregulation of the tryptophan-kynurenine pathway to inflammation and severity of COVID-19소변 대사체학은 트립토판-키누레닌 경로의 조절 장애를 COVID-19의 염증 및 중증도와 연결합니다Article Published on 2022-06-152022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), MERS, SARS, 바이오마커, 유전자 메커니즘, 치료기술, 치료법, [키워드] 3-hydroxykynurenine alteration Amino acid amino acids association Biomarker Biomarkers cause changes in Control coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 severity Critical disease severity dysfunction dysregulation healthy controls; Host immune response Increased Inflammation investigated Kynurenine kynurenines metabolism metabolite metabolomic profiling metabolomics Neutral pathway patients hospitalized performed proline proximal proximal tubule Quantitative SARS-CoV-2 SARS-CoV-2 infected patients SARS-COV-2 infection serum severity severity of COVID-19 Support systemic inflammation Transport Tryptophan Urine with COVID-19 [DOI] 10.1038/s41598-022-14292-w PMC 바로가기 [Article Type] Article
Proline-specific peptidase activities (DPP4, PRCP, FAP and PREP) in plasma of hospitalized COVID-19 patients입원한 COVID-19 환자의 혈장에서 프롤린 특이적 펩티다제 활성(DPP4, PRCP, FAP 및 PREP)Article Published on 2022-06-012022-09-11 Journal: Clinica chimica acta; international journal of cli [Category] MERS, 바이오마커, 진단, [키워드] Activation activities activity Alpha Biomarker changes in collected coronavirus COVID-19 COVID-19 patient COVID-19 patients decrease dipeptidyl peptidase Dipeptidyl peptidase 4 discharge DPP4 dysregulated dysregulation elevated FAP feature fibroblast Fibroblast activation protein alpha Follow-up healthy control healthy controls Hospital admission Hospital stay Hospitalization hospitalized COVID-19 patient hospitalized COVID-19 patients immune system investigated pathophysiological patients peptidase plasma PRCP PrEP proline Prolyl oligopeptidase Prolylcarboxypeptidase. Protein remained Sepsis Septic shock significantly significantly increased significantly lower utility were measured while [DOI] 10.1016/j.cca.2022.03.005 PMC 바로가기 [Article Type] Article
Addition of arginine hydrochloride and proline to the culture medium enhances recombinant protein expression in Brevibacillus choshinensis: The case of RBD of SARS-CoV-2 spike protein and its antibodyarginine hydrochloride와 proline을 배지에 첨가하면 Brevibacillus choshinensis에서 재조합 단백질 발현이 향상됩니다: SARS-CoV-2 스파이크 단백질의 RBD 및 그 항체의 경우Article Published on 2022-06-012022-09-11 Journal: Protein expression and purification [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] 96-well plates addition analysis anti-SARS-CoV-2 antibody antibody arginine arginine hydrochloride B.1.1.529 Bacterial bacterial cell bacterial cells bacterial strains Brevibacillus Cell condition contribute CoV-2 Culture culture medium demonstrated ENhance Escherichia coli eukaryotic eukaryotic cell exhibited expressed expression Fab functional functional expression gram-positive bacterium High-throughput expression and purification indicated medium Omicron variant physicochemical produced producing proline Protein protein expression Proteins purification RBD RBD of SARS-CoV-2 RBD. Receptor binding domain recombinant proteins reported SARS-CoV-2 spike protein secrete Spike protein strain the SARS-CoV-2 wild-type [DOI] 10.1016/j.pep.2022.106075 PMC 바로가기 [Article Type] Article
The mutational landscape of SARS-CoV-2 variants diversifies T cell targets in an HLA-supertype-dependent mannerSARS-CoV-2 변이체의 돌연변이 환경은 HLA 수퍼타입 의존 방식으로 T 세포 표적을 다양화합니다Article Published on 2022-02-162022-09-11 Journal: Cell Systems [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] analyzed CD8 country COVID-19 delta variant dominant driven by epitope Epitopes expressing HLA HLA-B HLA-B allele HLA-B alleles Immunity immunodominant individual mass vaccination mutants Mutation pandemic peptide population level predicted prevalent proline proteome residue RNA SARS-CoV-2 SARS-CoV-2 genome assemblies SARS-CoV-2 variant supertype T cell T cell epitope T cell epitopes target vaccination variant Variant. variants [DOI] 10.1016/j.cels.2021.09.013 PMC 바로가기 [Article Type] Article
Broad-Spectrum Antiviral Activity of the Amphibian Antimicrobial Peptide Temporin L and Its AnalogsArticle Published on 2022-02-132022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, COVID19(2023년), SARS, [키워드] activity addition Amphibian AMPs analyzed antimicrobial antimicrobial peptide antimicrobial peptides Antiviral antiviral activity antiviral agent Antiviral effect antiviral therapy Compound Coronaviruses COVID-19 pandemic cytotoxicity demonstrated DNA and RNA drug drug resistance evaluated evidenced frog peptides Glutamine Gram-negative bacteria help herpesviruses HSV-1 Influenza virus inhibit inhibition activity MOST overcome peptide peptides previous study Pro proline reduced replaced Replication rising SARS-CoV-2 selective Temporin temporins Therapeutic molecule therapeutic option Threats viral family virus Virus-host interaction viruses widespread [DOI] 10.3390/ijms23042060 PMC 바로가기 [Article Type] Article